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By:
Katie Morrison
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Decks in this class (41)
lecture 1 - polarity
Polarity,
H bonds,
Liquid water structure
10
cards
lecture 2 - amino acids
Glycine 3 letter,
Glycine 1 letter,
Gly
92
cards
lecture 3 - peptides
What is a peptide,
Condensation reactions,
Hydrolysis reactions
22
cards
lecture 4 - purification by solubility
Definition of purification,
What properties can we use,
Purification by solubility depend...
16
cards
lecture 5 - purification by chromatography
Types of chromatography 4,
Ion exchange what is the solid ph...,
2 types of ion exchange chromatog...
10
cards
lecture 6 - assessing purity with SDS-PAGE
Step 1 to assess purity and yeild,
Purity,
Yeild
16
cards
lecture 7 - purification with isoeclectric focusing
How does isoelectric focusing ief...,
Ief is typically combined with wh...,
How do we interpret the sds page ...
5
cards
lecture 8 - quantifying purity
Spectophotometry,
Beer lambert law,
B l is useful for determining the...
19
cards
lecture 9 - intro to bioinformatics
Why do we need bioinformatics 3,
Is dna sequencing easier or harde...,
What are the post translational m...
9
cards
lecture 10 - Edman degradation
Is edman degradation repetitive,
Where does edman degradation start,
What is the edman reagent what do...
14
cards
lecture 11 - sequencing long polypeptides
List 4 limitations of dna sequencing,
How do we sequence long sequences,
Where does trypsin cut
13
cards
lecture 12 - disulphide bonds
What is a disulphide bond,
How do we prepare a protein with ...,
What is a con of how we sequence ...
4
cards
lecture 13 - mass spectrometry
Why is mass spectrometry useful 3,
What is mass spectromoetry actual...,
How does measuring mass by mass s...
15
cards
lecture 14 - bioinformatics: homologous evolution
How do we use bioinformatics info 4,
Define homologous,
What do homologous sequences imply
17
cards
lecture 15 - protein structure: peptide bonds
What are the general themes for p...,
Where does a peptide bond occur,
What is the configuration of a pe...
14
cards
lecture 16 - protein structure: cis vs trans peptide bonds
For a trans peptide bond,
For a cis peptide bond,
Between cis or trans which is mor...
4
cards
lecture 17 - protein structure: alpha helix
What composes the 2 structure of ...,
Explain the helix,
How does the helix occur
12
cards
lecture 18 - protein structure: beta sheets
Explain beta sheet structure,
What is a beta sheet stabilized by,
Explain anti parallel h bonds bet...
8
cards
lecture 19 - protein structure: loops & turns
Explain what loops and turns do,
Do loops and turns have repetitiv...,
Are loops flexible why or why not
9
cards
approximate pKr values
What are the ionizable amino acids,
Pkr for tyr,
Pkr for cys
10
cards
lecture 20 - tertiary & quaternary structure
Explain the tertiary structure,
What is the tertiary structure st...,
Define long range interactions
14
cards
lecture 21 - fibrous proteins & alpha keratin
Explain the structure of fibrous ...,
Fibrous proteins are usually comp...,
How does the tertiary structure o...
15
cards
lecture 22 - collagen
What does collagen form,
What is the main function of coll...,
Explain the primary structure of ...
10
cards
lecture 23 - fibroin and fibrous proteins summary
Fibroin is commonly reffered to as,
What are the characteristics of f...,
Explain the primary structure of ...
10
cards
lecture 24 - globular protein folding
Explain general characteristics o...,
Explain generally how we study gl...,
Explain the process of heat degra...
13
cards
lecture 25 - globular protein misfolding diseases
List examples of globular protein...,
Explain amyloids,
What enhances the likelihood of f...
5
cards
lecture 26 - intrinsically disordered globular proteins
Question what sort of aa composit...,
Explain the primary structure of ...,
Explain the functions of intrinsi...
6
cards
lecture 27 - structure and function of globular proteins
How do we study structure and fun...,
What conservative substitutes lik...,
A lack of conservation at certain...
9
cards
lecture 28/29/30 - immunoglobulin structure
Define a family of structures,
Although they are a family each i...,
How diverse is the fam of immunog...
23
cards
lecture 31 - glycan binding proteins - GAG proteins
What do glycan bidning proteins bind,
What are the two groups of glycan...,
Describe gag proteins
17
cards
lecture 32-34 - glycan binding proteins - lectins
What do lectins bind with what af...,
Describe n glycans,
Describe o glycans
37
cards
lecture 35/36 - ice binding proteins
What are the two groups of ice bi...,
What do all afps afgps bind,
Are afps diverse describe their e...
24
cards
lecture 37-41 - myoglobin/hemoglobin
Do o2 binding proteins bind rever...,
What are o2 binding proteins invo...,
Does o2 have low or high solubility
58
cards
lecture 42 - enzymes intro
What are two key factors of enzym...,
List the 4 properties of catalyti...,
Describe how catalytic enzymes al...
16
cards
lecture 43 - general catalysis mechanisms
Generally what are two ways in wh...,
Explain the gibbs free energy gra...,
G for the uncatalyzed conversion ...
14
cards
lecture 44 - enzyme kinetics
What do enzyme kinetics help us u...,
What is the equilibrium constant ...,
If we were to plot product format...
17
cards
lecture 45 - enzyme inhibition kinetics
Where does enzyme inhibition typi...,
Can there be reversible enzyme in...,
Describe competitive enzyme inhib...
16
cards
lecture 46 - enzyme catalysis mechanisms
Describe the catalytic relationsh...,
What are the three modes of catal...,
Describe acid base catalysis
20
cards
lecture 47 - transition state stabilization and substrate recognition
What is the primary way in which ...,
Give a general reaction for the c...,
Are tetrahedral intermediates hig...
19
cards
lectures 48-51 - glycosyltransferases and glycosylhydrolases
What do we call the bond that hol...,
What are the two ways in which we...,
What is the anomeric carbon
29
cards
winter midterm
Glycosidic bond and composition o...,
Which of the enzymes is incorrect...,
What does glucagon upregulate
25
cards
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