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Negin rezaie
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Decks in this class (28)
DNA Structure
Pyrimidines,
Purines,
Nucleoside
23
cards
Manipulation of DNA
What are the physical properties ...,
Solubility of dna,
Viscosity of dna
20
cards
The mechanism of DNA replication
Plausible models for dna replication,
Semiconservative,
Conservative
27
cards
The replication of (microbial and) eukaryotic genomes
High fidelity of dna replication,
Error in dna replication,
Tautomeric forms
28
cards
Bacterial plasmids and transfer of genetic material
Sizes of genomes,
Viruses have a large diversity in...,
Eukaryotic genomes nuclear
29
cards
Probes, DNA amplification and genomic libraries
Detection of a specific gene,
What are the pcr minimum requirement,
What is the problem in pcr and wh...
24
cards
Recombinant DNA techniques
What dna need to fragmented,
Restriction enzymes,
Why do bacteria make restriction ...
20
cards
PCR technique
Denaturation,
Annealing,
Extension
19
cards
DNA sequencing and the Human Genome project
What is required for sequencing o...,
Dna sequencing by the chain termi...,
Difference between dideoxyribonuc...
17
cards
Transcription l
The biological role of transcription,
Is rna molecule a single strand h...,
What is coding strand and what is...
29
cards
The Genetic Code
The central dogma of molecular bi...,
Unit of code specifying a single ...,
What is a codon how many possible...
12
cards
Translation (l)
Which types of rna is most abunda...,
Structure of ribosomal rna,
The molecular organisation of the...
17
cards
Translation (ll)
Implication of antibiotics,
Can the antibiotic that block tra...,
Which antibiotic effect the small...
7
cards
Pre-lab questions
The design of the specific primer...,
What is the melting temperature i...,
Isozymes are enzymes that select ...
29
cards
Lab 7 - Control of GFP gene expression
What is an operon,
Are genes within an operon under ...,
Lac operon
29
cards
Lab 8-Plasmid Isolation
What is centrifugation,
How centrifugation is performed i...,
What is pellet and supernatant
12
cards
Regulation of transcription in bacteria I
Why not all genes are expressed a...,
What determines the different typ...,
Eukaryotic cell have off ground s...
32
cards
Regulation of transcription in bacteria II
What is an inducible gene,
The gene encoding for biosyntheti...,
Why is there an inducible gene
14
cards
Transcription in eukaryotes and gene repression by noncoding RNAs
Mrna synthesis and processing in ...,
What happens to prokaryotic mrna ...,
7 methylguanosine cap at 5 end
32
cards
Splicing
Where does the processing of mrna...,
What is splicing,
What are introns
24
cards
Chromatin remodelling and modification
Why eukaryotic gene are off in gr...,
Chromatin structure can be altere...,
Packaging of eukaryotic dna into ...
24
cards
Regulation of protein activity
Cell identity is defined by its,
What is proteome,
Regulation is effected at multipl...
34
cards
Mutation
Spontaneous occur naturally,
Induced require a mutagen,
Tautomeric forms of dna basesspon...
21
cards
DNA Repair: Damage
Genetic stability,
Dna repair is critical,
Human syndromes linked to defecti...
31
cards
DNA Recombination: Double Stranded Breaks
Double stranded breakscause and p...,
What happens if double stranded b...,
Double stranded break repairtwo m...
32
cards
DNA recombination and the immune system
Immune cells activate adaptive im...,
Site of development of b and t cells,
Bcr antibodies and tcr see differ...
30
cards
DNA recombination and cancer
The bcr ig constant c gene segmen...,
Different isotypes are generated ...,
First anti body secreted
27
cards
DNA Recombination for Genetic Engineering
Naturally occurring genetic mutat...,
Cystic fibrosis is caused by,
X linked hyper igm syndrome is ca...
23
cards
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BMS1062-
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