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J Hamel
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Decks in this class (25)
Introduction
What is genetic analysis,
What is a phenotype,
What is a genotype
14
cards
Mutations
What is a mutation,
What is a wild type allele,
What are 4 ways to classify mutat...
25
cards
Classifying mutations
What are 3 ways to classify mutat...,
What is a base substitution,
What are the two types of base su...
14
cards
Muller's morphs
How are mutations classified in m...,
What is a compound heterozygote,
What is the df allele
35
cards
Mutant hunts - introduction
What is a mutant hunt,
What are the 4 motivations for do...,
What is the first step in a mutan...
15
cards
Balancer chromosomes
How do balancer chromosomes stop ...,
What happens if a homologous chro...,
How do we recognize that an organ...
46
cards
Pulsed Field Gel Electrophoresis
What is the advantage of using pf...,
How do you prepare a sample to us...,
How do you run a pfge gel
4
cards
Screens vs selections
What is a screen,
What is a selection,
If we could do either would it be...
4
cards
Saturation
What is the law of diminishing re...,
What is the divergence point,
How do we determine if we are at ...
8
cards
Complications with mutant hunts
What are 5 complications with mut...,
What is incomplete penetrance,
How does incomplete penetrance co...
8
cards
Mapping mutations
How do we choose a mapping allele...,
What is the genotype of the teste...,
Which generation do we look at to...
35
cards
Mapping with molecular markers
Why would we use snps for mapping...,
What do we look for when mapping ...,
What criteria needs to be met whe...
21
cards
Polytene chromosomes
What are the 4 reasons why drosop...,
When do drosophila use polytene c...,
What process creates polytene chr...
33
cards
Complementation testing
Why do we do complementation testing,
What are allelic mutations,
What are non allelic mutations
10
cards
Yeast vectors
What are vectors,
Why are eukaryotic vectors based ...,
What is a vector called when it c...
44
cards
DNA libraries
What is a dna library,
What is a genomic dna library,
What is in a genomic dna library
19
cards
Yeast transformation
What are 4 types of selectable ma...,
How do we use nutritional markers...,
How do we use carbon source marke...
14
cards
Drosophila vectors and transformation
What are most drosophila vectors ...,
What mechanisms do p elements use...,
What does an endogenous p element...
37
cards
C elegans transformation
How do you distinguish hermaphrod...,
Do we transform male or hermaphro...,
What type of transformation do we...
6
cards
Transformation of mammalian cells
What type of mammalian cells do w...,
What are the 6 methods to transfo...,
Which 3 transformation methods ar...
9
cards
Transgenic, knockin and knockout mice
What do we consider to be a trans...,
What is a transgenic mouse,
What is a knockout mouse
16
cards
Cloning a gene
How do you do cloning by compleme...,
If we dont get a transformant wit...,
What is another name for cloning ...
35
cards
Gene to phenotype
What is site directed mutagenesis,
Why is site directed mutagenesis ...,
What does site directed mutagenes...
29
cards
Genome editing
What are the 2 double strand brea...,
What is the problem with ends out...,
Why cant we use restriction enzym...
19
cards
Using one gene to find another
What are 4 methods to identify ot...,
How does a yeast two hybrid assay...,
What are the 2 plasmids used in a...
23
cards
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Genetic Analysis of Eukaryotes
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