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Decks in this class (14)
1-Protein quantification: the Bradford assay
How to come up with an experiment 1,
What are qualitative quantitative...,
Which assay can be both qualitati...
24
cards
2-Endonuclease restriction analysis
Characteristics of a plasmid 1,
What is the principle vector used...,
What is a vector 3
35
cards
3 - PCR to determine gene orientation in plasmids
Psychrophiles 1,
Psychrotrophs 2,
Mesophiles 3
16
cards
4 - preparation of comeptent cells & transformation of E.coli with plasmid DNA
What is the transformation of bac...,
What are competent cells 2,
What are the different methods to...
25
cards
5 - purification, electrophoresis & transfer of GST fusion proteins
What are the basic steps for gene...,
What are the 3 major components o...,
What is the expression vector cho...
22
cards
6 - immunodetection of GST fusion protein
Immunoblot analyses 1,
Incubation with antibodies 2,
Alkaline phosphatase detection me...
14
cards
midterm practice:
Why are restriction enzymes so us...,
If you had the privilege to disco...,
Name the three types of dna ends ...
12
cards
7 - overview and plaque assays
What bacteriophages are we lookin...,
What is our goal in this module 2,
2 key players in this module 3
16
cards
8 - generating BIMs
What is the plaque assay efficien...,
Why are bims resistant to phage i...,
What are the different ways abcte...
21
cards
9 - Characterizing BIMs and CRISPR-Cas System
What are the crispr loci in smthb...,
Which protospacer is most effecti...,
How is a new spacer integrated in...
18
cards
10 - CRISPR-Cas system and phage mutation
What are the 3 steps in crispr ca...,
Whats different in spacer acquisi...,
How to have efficient adaptation 3
19
cards
11- genome editing and DNA extraction
Why do we use spot test 1,
When is the 30 300 plaque rule us...,
Where does cas9 cut 3
21
cards
12 - Analysis of sequences and conclusion of lab
Goal of lab 12 1,
What should we identify 2,
Where is located the newly acquir...
8
cards
Final review (7-12)
How many endonucleases does s the...,
Sapcers are trasncribed into sepa...,
The 2 requirements before cas9 ca...
14
cards
More about
MIMM 384
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