Techniques Course

This class was created by Brainscape user emily chetwin. Visit their profile to learn more about the creator.

Decks in this class (22)

Isolation Of Human Genomic DNA
Main steps in dna isolation,
What needs to be considered when ...,
Role of sucrose
13  cards
Principles Of Cloning
Why do we clone,
Methods of transfer of genetic ma...,
Applications of cloning
20  cards
Amplifying The Blueprints Of Biology
Pcr steps,
Pcr reagents,
Tm
7  cards
Sequencing
Sanger sequence overview,
Requirements for dna replication,
How do ddntps differ
11  cards
Gene Regulation And Expression
Levels at which gene expression i...,
Structure of rna,
Effect of dna methylation
16  cards
Protein Methods
Requirements for protein purifica...,
Value of protein purification,
Basic scheme of protein purification
3  cards
Protein Purification: Chromatography
Equipment for liquid chromatography,
Types of chromatography,
Definition of amphoteric
5  cards
Classical biochemistry Approaches To Studying Proteins
Advantage of native proteins,
Native proteins,
Disadvantages of native proteins
11  cards
Into To Mass Spec
Mass spectrometers are able to,
Definition of mass spec,
Mass spectrometers are unable to
14  cards
Intro To Proteomics
Problems with 2de ms,
Challenges in proteomics,
General ms based proteomics workflow
10  cards
Protein Fluorescence
Why do we use fluorescence,
What is fluorescence,
Things that can be measured with ...
8  cards
Isothermal Titration Calorimetry
Attractive features of itc,
Definition of itc,
Limitations of itc
7  cards
How To Study Proteins By X-ray Crystallography
How do crystals form,
General workflow,
Conditions that crystal formation...
8  cards
Cell Culture
Classification by manner of growth,
Types of culture and cell lines,
How to sub culture
3  cards
Basic Principles Of Histological Staining
Disadvantage of h e,
How to preserve tissue,
Disadvantages of acid base dyes
4  cards
Basic Principles Of Microscopy
In phase amplitude out if phase a...,
Abbes law,
Components of a microscope
7  cards
Fuorescence And Confocal Microscopy
Explain stokes shift,
Advantages of fluorescence micros...,
Problems with fluorescence micros...
9  cards
Basics Of Multiphoton, Superresolution And Structures Illumination Microscopy
Sr sim,
Examples of super resolution methods,
Sted
6  cards
CyTOF
How is the plasma created,
Basic steps in cytof,
3 chambers of the torch
8  cards
Epigenetics
Technologies in epi genomic research,
Definition of epi genetics
2  cards
Circular Dichroism
Definition of cd
1  cards
NMR
How to determine structure with nmr,
What can nmr do
2  cards

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Techniques Course

  • Class purpose General learning

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