1.

Question 1

Aseptic Technique steps 1-7

Answer 1

1. Wipe down bench space with alcohol
2. Connect and turn on Bunsen burner
3. Light Bunsen burner
4. Sterilize loop by holding to flame. DO NOT PUT ON BENCH WHILE IN USE. If placed on bench, flame again when ready to use
5. Keep all open cultures within 6 inches of the flame.

6.When done, quickly pass culture tube opening through flame, then cap

7.Once all work is done, turn off Bunsen burner, and clean bench with Bacdown and alcohol

Question 2

Taking a sample from one culture and moving it to a fresh culture medium. This is called?

Answer 2

subculturing

Question 3

List the 3 reasons why subculturing is done

Answer 3

1. Make more of a culture
2. Keep a culture going longer
3. Introduce culture to experimental mediums

Question 4

How do you do a broth-to-broth culture? list the steps (1-5)

Answer 4

1. Sterilize loop. Let cool.
2. Gently place loop in broth to pick up some culture
3. Remove loop from culture and place in fresh nutrient broth. Gently shake to dislodge culture
4. Cap both cultures
   5.Sterilize loop

Question 5

Slant to broth culture

Answer 5

1. Sterilize loop. Let cool.
2. Gently scrape top of agar to gather culture on loop
3. Place loop in fresh nutrient broth. Gently shake to dislodge culture
4. Cap both cultures
5. Sterilize loop

Question 6

Broth to slant culture

Answer 6

1. Sterilize loop. Let cool.
2. Gently place loop in broth to pick up some culture
3. Remove loop from culture
4. Gently rub loop on top of agar in a zig-zag pattern.
5. Cap both cultures
   6.Sterilize loop

Question 7

Inoculating nutrient deep agar is also called

Answer 7

“Stab cultures”

Inoculate your loop as previously described, then carefully stab it into the center of the agar
When done, cap both tubes and sterilize loop

Question 8

What is the reason for a four-way streak?

Answer 8

• Done to isolate bacteria colonies
• Can be done in a circular or quadrant method (see below)
• Performed same way as an agar slant culture