(10) Clin Corr: EIC

Question 1

What happens during EIC?

• Can dogs die from this?

Answer 1

• hind limbs become weak, elevated rectal temperatures
• yes, but not often

Question 2

What is a normal lab’s temp after exercise? why so high?

What does this mean?

Answer 2

• 105 - 108
• no sweat glands
• dogs that get EIC really aren’t any higher than normal dogs during exercise

Question 3

Is EIC myasthenia gravis? What is myasthenia gravis? How do we know?

Answer 3

• no, antibodies against ACh receptors, dogs with EIC didn’t have measureable AChR antibodies

Question 4

Is it same mutation (RYR1) that causes malignant hyperthermia in dogs?

What does this mutation cause in pigs?

Answer 4

• no
• porcine stress syndrome

Question 5

Were there significant differences in blood chemistry, blood oxygen, EKG, and muscle pathology between normal dogs and dogs with EIC?

Answer 5

no

Question 6

What gene was the EIC mutation found on?

Which gene specifically?

What was the specific nucleotide change?

Amino acid change?

Answer 6

• CFA9
• Dynamin 1
• G changed to T at 767
• codon 256 from arginine (basic/polar) to leucine (non-polar)

Question 7

What does dynamin 1 do?

Where is it expressed?

What takes over for the lack of dynamin 1 doing its job?

Answer 7

• dynamin 1 is involved in vesicle recycling
• CNS, spinal cord
• dynamin 2 and 3 can pick up a bit of the slack - but not all of it

Question 8

What’s up with the shibire? What does it tell us about EIC?

Answer 8

• Shibire flies have all of their dynamin genes knocked out - they experience similar side afects when the temperature is raised - and then recover like dogs when it goes back down - this could indicate that EIC is temperature dependent

Question 9

• Do dogs with EIC have progressive disease? Can they live normal lives?

What drug has been shown to work a little bit? How does it work? Does it target the problem directly?

Answer 9

• no, yes as long as they avoid prolonged physical activity
• phenobarbital, it works by decreasing level of excitement, does not address the issue directly

Question 10

How is EIC different from heat stroke?

Answer 10

• heat stroke collapse takes hours to days for recovery, not the case with EIC!

Question 11

How is the whole breeding problem being addressed?

Answer 11

• decrease carriers, but don’t do it altogether - breed and replace

Question 12

How is EIC tested?

Answer 12

• restriction fragment length polymorphism

Question 13

What type of mutation is it?

Answer 13

• aautosomal recessive inheritance