Gene expression definition
where genetic information of a gene of DNA is transcribed and translated to synthesise products such as proteins.
Three types of RNA
mRNA
tRNA
rRNA
mRNA definition
The type of RNA that is involved in transcribing a gene of DNA and transporting it from the nucleus to the ribosome.
tRNA function
tRNA carries an amino acid to the ribosome in translation to bind with mRNA
rRNA function
To be part of the ribosome
Triplet definition
Three bases of RNA
Codon definition
A triplet of bases on mRNA that code for an amino acid when mRNA binds with tRNA
Anti Codon
Three bases of tRNA which bond with mRNA.
Differences between RNA and DNA
RNA is single stranded whereas DNA is double stranded
RNA contains ribose sugar whereas DNA contains deoxyribose sugar
RNA contains Uracil whereas DNA contains thymine
Transcription stage 2
Free RNA nucleotides approach the unzipped DNA strands.
Order of gene expression
DNA
Transcription
mRNA
translation
tRNA
Ribosome
Protein
Transcription definition
The process of making an RNA copy of a gene of DNA
Transcription stage 1
DNA unwinds and RNA polymerase unzips the molecule by breaking apart the weak and temporary hydrogen bonds, exposing the bases.
Transcription stage 3
Free RNA nucleotides find the complimentary bases on the DNA strand
Stage 4 of Transcription
Weak and temporary hydrogen bonds form between the bases of DNA and the bases of the RNA free nucleotides.
Stage 5 of transcription
The enzyme RNA polymerase bonds together the sugar phosphate backbone of the RNA strand
Stage 6 of transcription
Weak and temporary hydrogen bonds break between the DNA and RNA, and then an mRNA primary transcript strand is formed, DNA redwinds into a double stranded helix.
Stage 7 of transcription
The mRNA primary transcript undergoes RNA splicing, this is where introns which are non coding regions of RNA are removed from the mRNA strand and the exons, which are coding regions of RNA are compacted together, making the mature transcript strand small enough to leave the nucleus.
Stage 1 of translation
The mRNA strand leaves the nucleus and travels to the ribosome.
Stage 2 of translation
The tRNA strand collects an amino acid and travels to the ribosomes to bond with mRNA
Stage 3 of translation
The codon of mRNA bonds with the anti codon of tRNA using complimentary base pairing.
Stage 4 of translation
Peptide bonds form between the adjacent amino acids, forming a polypeptide chain.
Stage 5 of translation
The tRNA strand will then collect another amino acid to repeat the process.
Alternative splicing definition
Where a different set of exons are spliced together forming a different mature transcript and a different protein in RNA splicing.