1.3 Path

Question 1

What is the general stain used in path?

Answer 1

H&E Stain

Question 2

What is the H&E stain?

Answer 2

a general stain used to differentiate the nucleus and major cellular compartments
hematoxylin: acidic components blue/purple
nuclei
eosin: basic components pink
cytosol, proteins

Question 3

What is the trichrome stain? What is it used for?

Answer 3

Multiple techniques used, but generally 3 colors
mainly to highlight collagen fibers
increased CT deposition with fibrosis, related conditions

Question 4

What is prussian blue used for?

Answer 4

used to detect intracellular iron deposits, ferritin and hemosiderin
increased in active macrophages, bi-product of RBC breakdown, hemolysis, trauma