15. DNA Replication

Question 1

Meselson and Stahl

Answer 1

Labeled nitrogen and looked at where it ended up–found semi-conservative replication

Question 2

Necessary Components for Replication Initiation (4)

Answer 2

1. Free nucleotide base
2. Free 3’ end with O on backbone ribose
3. NTP (Adds base and phosphate, releases 2 phosphates)
4. DNA polymerase to catalyze 3’-5’ phosphodiester bond

Question 3

Classes of Polymerases (4)

Answer 3

DNA Dependent DNA Polymerase (Replication)
DNA Dependent RNA Polymerase (Transcription)
RNA Dependent DNA Polymerase (Retroviruses)
RNA Dependent RNA Polymerase (Replicase viruses)

Question 4

Necessary Components for DNA Polymerase Function (3)

Answer 4

1. 3’ end with O on backbone deoxyribose
2. Unimpeded nucleotide base on template
3. Correct NTP

Question 5

DNA Polymerases

Answer 5

I: Error correcting (slow)
II: Unknown
III: Primary Replication

Question 6

Nick

Answer 6

A missing phosphodiester bond

Question 7

Gap

Answer 7

Missing nucleotides