2. Principles

Question 1

What was one of the key findings of the Mega Mouse Study?

Answer 1

The National Center for Toxicological Research (NCTR) performed a large dose-effect study of AAF designed to estimate the effective dosage producing a 1.0% tumor incidence in mice (i.e. the ED01)

This finding highlights the mutagenic potential of ENU.

Question 2

What precedent did the Mega Mouse Study set?

Answer 2

For large-scale animal studies in genetic toxicology and risk assessment

The study’s methodology has influenced future research in the field.

Question 3

therapeutic index

Answer 3

LD50/ED50

Question 4

Margin of safety

Answer 4

LD1/ED99

Question 5

Benchmark dose

Answer 5

measure of min tox response such as 10%

Question 6

MOS (margin of safety) / MOE (margin of exposure)

Answer 6

daily exposure/TD10

Question 7

Clinical chemistry

Answer 7

glucose, creatinine, BUN (blood urea nitrogen), sodium, potassium, CO₂, chloride, calcium, phosphorus, cholesterol, triglycerides, bilirubin, AST (SGOT), ALT (SGPT), iron, total protein, albumin, globulin