4.1 Lab safety

Question 1

Potential that a chosen action will lead to an undesirable out come

Probability of harm form occurring given a specific situation

Answer 1

Risk

Question 2

Has the potential for causing harm

Answer 2

Hazard

Question 3

Risk = Hazard + ______

Answer 3

Situation / action

Question 4

Types of hazards

Answer 4

Safety / physical
Chemical
Radiological
Biological
Ergonomic
Socio-Cultural

Question 5

Posture / proper height of stuffs like chair etc.

Answer 5

Ergonomic

Question 6

Environment/Animal test lab

Stigma for animal testing

Beliefs about animal testing

(Cultural aspect on what you believe in Like cloning) - Does it make you uncomfortable doing this that may hinder your mentality or your reputation?

Bullying

Answer 6

Socio-cultural

Question 7

Potential source of harm cause by biological materials

Answer 7

Biological hazard

Question 8

Any material composed of, containing or that may contain biological agents

Answer 8

Biological material

Question 9

Any microbial entity, cellular or non-cellular that may able to produce infection

Answer 9

Biological agents

Question 10

Are infections, either symptomatic or asymptomatic, that are acquired through lab or lab-related activities, as a result of working with infectious agent

Answer 10

Laboratory acquired infections

Question 11

LAI how many percent due to equipment failure

Answer 11

20%

Question 12

LAI how many percent due human factors lacking
- PPE
- Policies

Answer 12

80%

Question 13

What are the top 4 accidents resulting to infections?

Answer 13

1. Spills and splashes
2. Needle and syringe pricks
3. Sharp objects, broken glass
4. animal scratch or bite

Question 14

Containment to prevent UNINTENTIONAL accidental release of pathogens

Protects people from bad bugs

Answer 14

Lab biosafety

Question 15

Prevent loss, theft or INTENTIONAL release of pathogens and toxins

Protects bad bugs from bad people

Answer 15

Lab biosecurity

Question 16

What are the 3 purpose of biosafety?

Answer 16

Protect
- users
- Those outside the Lab
- Environment

Question 17

Constituting the national Committee on Biosafety on the Philippines and for other purpose

Answer 17

EO no, 430 s 1990

Question 18

Constituting the various Biotechnology institutes within the University of the Philippines System as the Network of National Institutes of Biotechnology

Answer 18

Proclamation no. 526, s 1995

Question 19

Prescribing guidelines and establishing a Regulatory framework for Prospecting of biological and Genetic Resources, Their By-products and Derivatives, for Scientific and Commercial Purposes; and for Other Purposes

Answer 19

EO no 247. s 1995

Question 20

Establishing the National Biosafety Framework, Prescribing Guidelines for Implementation, Strengthening the National Committee on Biosafety of the Philippines, and for Other Purposes

Answer 20

EO no. 514 s, 2006

Question 21

We Shall promote the Safe and responsible use of modern biotechnology and its products as one of several means to achieve and sustain food security, equitable access to health services, sustainable and safe environment and industry development

Answer 21

Philippine National Policy Statement on Modern Biotechnology (2001)

Question 22

What are the 4 Biosafety principles?

Answer 22

1. Equipment safety
2. Levels of Protection
3. Practice and procedures
4. Facility Design and Construction

Question 23

Standard microbiological practices

Lab techniques

Answer 23

Practice and procedures

Question 24

Containment barrier
Minimize exposure to hazard
Engineering controls
PPE

Answer 24

Equipment safety

Question 25

Secondary barrier/engineering controls • Contributes to worker protection • Protects outside the laboratory

Answer 25

Facility Design and Construction

Question 26

Clean benches Air flow Filters (HEPA)

Answer 26

Biosafety cabinet (additional airflow - Keeps the bacteria inside) Laminar flowhood

Question 27

Meaning of HEPA

Answer 27

High efficiency particulate air filters

Question 28

Prevents fume from chemicals

Answer 28

Fumehood (Right pic)

Question 29

Whats the difference between between Biosafety cabinet and Laminar flowhood

Answer 29

Difference biosafety features

Question 30

High pressure inside the room than outside (Keeps the room clean - Clean room)

Answer 30

Positive pressure

Question 31

Low pressure in side than outside (Keeps the particulates inside - Dirty room)

Answer 31

Negative pressure

Question 32

Basic teaching research GMT Open work

Answer 32

Biosafety level 1

Question 33

Primary health services, diagnostic services, research GMT, PPE, Biohazard sign BSC for potential aerosols

Answer 33

Biosafety level 2

Question 34

Special diagnostic services Airborne bacteria Controlled access, Directional airflow

Answer 34

Biosafety level 3 (Containment)

Question 35

Dangerous pathogens (Ebola, Small pox, Etc,) Class III BSC

Answer 35

Biosafety level 4

Question 36

Not A recognized containment level in biosafety guidance documents Conducted in BSL 2 lab with Biosafety practices and procedures typically found at BSL 3

Answer 36

BSL 2+

Question 37

Assignment of microorganisms into groups based on: Pathogenecity, mode of transmission, preventive measures, treatment

Answer 37

Risk groups

Question 38

A microorganims that is unlikely to cause harm or disease No or low individual and community risk

Answer 38

Risk group 1

Question 39

A pathogen than can cause human or animal disease but no serious to lab workers Moderate individual risk, Low community risk

Answer 39

Risk Group 2

Question 40

Serious human or animal disease on one infected human (Blood borne pathogens) high individual risks, Low community risk

Answer 40

Risk group 3

Question 41

Serious disease that can spread to the community, treatment may not be available High on both individual and community risk

Answer 41

Risk group 4