A2.2 - microscopy practical activity (3d)

Question 1

how are temporary mounts of cells and tissues made?

Answer 1

1. a thin sample is taken and placed on a slide
2. stains may be used to improve visibility of structures
3. a cover slip is lowered gently over the sample, avoiding air bubbles and blotting excess stain

Question 2

how are light microscopes used to produce a focused image?

Answer 2

1. lamp is switched on and the lowest power objective lens is selected, with the stage being moved to the lowest position
2. place slide in middle of stage so light is shining through it
3. move stage to the highest position until the lens doesn’t touch the sample
4. look through eyepiece leans and use coarse focus and fine focus knob to focus the image
5. move sample if needed and use a higher magnification