1
Q
Non-competitive Inhibitor
A
- Very rare
- Inhibitor binds to free enzyme and ESC
- Lowers the # of free enzyme molecules
- Vmax reduced, Km not usually effected
2
Q
Lineweaver-Burk Plot equation (LWBP)
A
- Straight line graph
3
Q
Michaelis-Menten Graph + Equation
A
V = (Vmax.[S])/(Km + [S])
4
Q
Effect of Competitive inhibitor on LWBP
A
- Gradient increases
- Y intercept does not change
- X intercept (1/Km) gets smaller (because it is a reciprocal, so the bigger Km gets, the smaller the 1/Km gets)
5
Q
Effect of Uncompetitive inhibitor on LWBP
A
- Gradient is the same
- Y intercept (1/Vmax) increases
- X intercept (-1/Km) increases
6
Q
Effect of Non-competitive inhibitor on LWBP
A
- Gradient increases
- Y intercept (1/Vmax) increases
- X intercept does not change
