Affinity Chromatography

Question 1

Ligand

Answer 1

Chemical group is covalently attached to the beads in the column

Question 2

Proteins binding to ligand

Answer 2

Proteins have an affinity towards the ligand so they bind tightly

Question 3

Bound proteins

Answer 3

Eluted by the addition of high concentration salt or ligand

Question 4

Tag

Answer 4

Peptide or protein that binds a ligand with a high affinity and specificity that is fused to the gene encoding target protein