Archaea

Question 1

Habitats of Archea

Answer 1

very extreme environment

Question 2

The polyphasic approach to taxonomy uses these methods

Answer 2

• Phylogenetic analysis
• Phenotypic analysis (motility,capsule,virulence)
• Genotypic analysis (presence-absence of specific genes)

Question 3

what’s the method of MLST

Answer 3

• several housekeeping genes from a species are sequenced

- aligned to the respective sequences of other individuals of the same specie

Question 4

MLST has sufficient resolving power to distinguish?

Answer 4

btw very closely related strains

Question 5

ho does MLST works?

Answer 5

1-isolate DNA
2-Amplify 6-7
3- Sequence
4-Allele analysis
5-Compare with other strains and generate tree