Bacterial Identification

Question 1

Staphylococcos aureus Identification

• Arrangement:
• O2 Requirement:
• Hemolysis:
• Glucose utilization:
• Bacitracin:
• Furazolidone:
• Lysostaphin
• Microdase:
• Catalase:
• Coagulase:
• DNAse:
• Colonies:

Answer 1

• Arrangement: Grape-like in clusters
• O2 Requirement: Facultative anaerobe
• Hemolysis: Beta hemolysis
• Glucose utilization: Fermentation
• Bacitracin: Resistant
• Furazolidone: Susceptible
• Lysostaphin: Susceptible
• Microdase: Negative
• Catalase: Positive
• Coagulase: Positive
• DNAse: Positive
• Colonies: Golden yellow colonies

Question 2

Micrococci Identification

• Arrangement:
• O2 Requirement:
• Hemolysis:
• Glucose utilization:
• Bacitracin:
• Furazolidone:
• Lysostaphin
• Microdase:
• Catalase:
• Coagulase:

Answer 2

• Arrangement: Tetrads/Packets
• O2 Requirement: Obligate aerobe
• Hemolysis: Non-hemolytic
• Glucose utilization: Oxidation (open tube +)
• Bacitracin: Susceptible
• Furazolidone: Resistant
• Lysostaphin: Restant
• Microdase: Positive
• Catalase: Positive
• Coagulase: Negative

Question 3

S. epidermidis Identification

• Catalase:
• Coagulase:
• Novobiocin:
• BAP:

Answer 3

• Catalase: Positive
• Coagulase: Negative
• Novobiocin: Susceptible (>16 mm)
• BAP: Sticky white

Question 4

S. saprophyticus Identification

• Catalase:
• Coagulase:
• Novobiocin:
• BAP:

Answer 4

• Catalase: Positive
• Coagulase: Negative
• Novobiocin: Resistant (<16 mm)
• BAP: Large yellow/orange/white

Question 5

Identification of Group A - S. pyogenes

• Bacitracin:
• SXT:
• PYR Test:
• CAMP Test:
• Hippurate Test:

Answer 5

• Bacitracin: Susceptible
• SXT: Resistant
• PYR Test: Positive
• CAMP Test: No enhanced hemolysis
• Hippurate Test: Negative

Question 6

Identification of Group B - S. agalactiae

• Bacitracin:
• SXT:
• PYR Test:
• CAMP Test:
• Hippurate Test:
• Bile Esculin:
• Growth in 6.5%:

Answer 6

• Bacitracin: Resistant
• SXT: Resistant
• PYR Test: Negative
• CAMP Test: Arrowhead hemolysis
• Hippurate Test: Positive
• Bile Esculin: Negative
• Growth in 6.5%: Positive

Question 7

Identification of S. pneumoniae

• Optochin:
• Bile Solubility:
• Inulin Fermentation:
• Animal inoculation:
• Neufeld quellung’s test:

Answer 7

• Optochin: Susceptible
• Bile Solubility: Soluble
• Inulin Fermentation: Positive
• Animal inoculation: Positive
• Neufeld quellung’s test: Capsulaw swelling

Question 8

Identification of Viridans Streptococci

• Optochin:
• Bile Solubility:
• Growth in 6.5% NaCl:
• Bile esculin:
• Inulin Fermentation:
• Animal inoculation:
• Neufeld quellung’s test:

Answer 8

• Optochin: Resistant
• Bile Solubility: Insoluble
• Growth in 6.5% NaCl: Positive
• Bile esculin: Negative
• Inulin Fermentation: Negative
• Animal inoculation: Negative
• Neufeld quellung’s test: Negative

Question 9

Identification of E. coli

• MAC:
• EMB:
• TSIA:
• IMVC:
• LOA:
• Motility:
• H2S:
• Urease:
• PAD:

Answer 9

• MAC: flat, dry with sorrounding dark pink area precipitated with bile salts
• EMB: Green metallic sheen
• TSIA: A/A
• IMVC: ++00
• LOA: ++0
• Motility: Positive
• H2S: Negative
• Urease: Negative
• PAD: Negative

Question 10

Identification of K. pneumoniae

• MAC:
• TSIA:
• IMVC:
• Motility:
• LOA:
• H2S:
• PAD:
• Urease:

Answer 10

• MAC: Mucoid colonies
• TSIA: A/A
• IMVC: –++
• Motility: Nonmotile
• LOA: +00
• H2S: Negative
• PAD: Negative
• Urease: Positive

Question 11

Identification of K. oxytoca

• MAC:
• TSIA:
• IMVC:
• Motility:
• LOA:
• H2S:
• PAD:
• Urease:

Answer 11

• MAC: Mucoid colonies
• TSIA: A/A
• IMVC: +-++
• Motility: Nonmotile
• LOA: +00
• H2S: Negative
• PAD: Negative
• Urease: Positive