Basics

Question 1

Reporting Criteria for Gram Stains

Answer 1

Rare = 10 organisms per oil immersion field

Question 2

Gram Stain Steps/Process

Answer 2

1. Crystal Violet (stain peptidoglycan)
2. Gram Iodine (traps CV)
3. Acetone (decolorize)
4. Safranin Red (counter stain)