1
Q
Step 1 PC
A
immunize rabbit
2
Q
2 PC
A
obtain blood sample
3
Q
3 PC
A
purify antigen using affinity chromatography
4
Q
Step 1 MC
A
Multiple immunizations
5
Q
2 MC
A
isolate B cells and tumor cells
6
Q
3 MC
A
Fuse B and tumor cells
7
Q
4 MC
A
Two sample: antigen specific and non-specific hybridoma
8
Q
5 MC
A
ID antigen specific by screening
9
Q
6 MC
A
clone antigen specific
10
Q
7 MC
A
results in monoclonal ABs
