biochem: lab techniques

Question 1

blot mnemonic

Answer 1

SNoW DRoP: Southern: DNA, Northern: RNA, Western: Protein. southwestern blot identifies DNA-binding proteins

Question 2

cre-lox system

Answer 2

inducibly manipulate genes at specific developmental points (e.g. to study a gene whose deletion causes embryonic death)

Question 3

RNA interference

Answer 3

fancy technique where dsRNA is complementary to target mRNA, causing degradation and decreased gene expression