Biochemical Tests

Question 1

What is the test for reducing Sugars?

Answer 1

Benedicts test

Question 2

Most sugars are known as reducing sugars and will give a positive result with the Benedict’s test. Which sugar is not a reducing Sugar?

Answer 2

Sucrose

Question 3

How do you perform the food test for reducing sugars?

Answer 3

1. Add 1 cm^3 of the test solution to a test tube
2. Add 1cm^3 of benedict’s solution
3. Heat >= 60 C using a water bath
4. look for red/brown precipitate
   reducing sugars react with copper sulphate

Question 4

What is a qualitative test?

Answer 4

Shows whether a particular substance is present (or not) but does not give an indication of now much is present

Question 5

Why is Benedict’s a semi quantitative test?

Answer 5

The colour of the precipitate gives an idea about the quantity of sugar present in the solution