Biochemistry Flashcards

(149 cards)

0
Q

The order and sequence of amino acid in a polypeptide determines what protein structure

A

Primary

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

The inhibition in a noncompetitive reaction

A

Binds simultaneously with substrate other that the active site

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Amino acids that cannot be synthesized in the organism are called

A

Essential amino acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Which hormone regulates the level of blood sodium

A

Aldosterone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Its a precursor of vitamin A

A

B-carotene

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q
Which of the ff is a precursor of vit D
Prostaglandin
Linoleic acid
Cholesterol
Aldosterone
A

Cholesterol

7-dehydrocholesterol -> cholecalciferol (vit D3)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Class enzymes introduces a double bond by the removal of hydrogen

A

Dehydrogenase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

The ionic property of amino acid is exhibited by its

A

Zwitterions form

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q
All of the ff are simple proteins except
Glutelins
Globulins
Albumins
Glycoproteins
A

Glycoproteins (proteins with oligosaccharides)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Simplest monosaccharide

A

Glyceraldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Denaturation of protein is a result of

A

Breaking of H bond

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Competitive inhibition is a ______ reaction

A

Reversible

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

In the seliwanoffs test, the reaction of resorcinol and acid on the sugar forms

A

Hydroxymethyl furfural

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

High conc of neutral salts caused the ppt of proteins. This is called

A

Salting out

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

The type of enzyme inhibition reaction whereby the inhibitor competes with the substrate at the active site

A

Competitive inhibition

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q
The ff are waxes except
Beeswax
Sperm oil
Bile acids
Lanolin
A

Bile acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

The inactive form of enzymes are called

A

Zymogens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Protein portion of the holoenzyme (enzyme with the cofactor)

A

Apoenzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q
Which of the ff has no alpha amino group
Proline
Hydroxyproline
Glycine
Both a and b
A

Both a and b contain imino group

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

An enzyme is a substance which

A

Act as a catalyst

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Milk curdling enzyme present in gastric juice of infants

A

Rennin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Carbohydrates are

A

Polyhydroxyaldehydes/polyhydroxyketones

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Insulin is usually classified as

A

Hormone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What amt of glucose is present in human blood

A

60-90mg in 100mL blood

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
It is the organelle which serves as the site of the electron transport chain
Mitochondria
25
The end product of the hydrolysis of glycogen is
Glucose
26
Iodine test is a reaction which may be used to identify carbohydrates. The reaction is due to
Presence of amylose portion
27
Benedicts reagent yield positive result to
Reducing sugars
28
Hypertonic solns will cause the cell to
Shrink
29
Rancidity of fats maybe due to
Oxidation
30
The deficiency of this hormone causes diabetes mellitus
Insulin
31
The active proteolytic enzyme in gastric juice
Pepsin
32
The site of oxidation reaction in electron transport chain is in the
Mitochondrion
33
Protein digestion atrts in the
Stomach
34
The conversion of an amino acid to sugar is
Gluconeogenesis
35
``` Which of the ff is not an amino acid Leucine Choline Valine Glycine ```
Choline is a nitrogenous base
36
The protein part of the enzyme molecule is the
Apoenzyme
37
Optimum temperature for enzyme activity in the body
37C
38
Glucose is stored in the liver as
Glycogen
39
The enzyme conformation adapts to the incoming substrate in
Induced fit theory
40
The process of converting glucose into glycogen is called
Glycogenesis
41
``` All are pyrimidine bases except Guanine Cytosine Uracil Thymine ```
Guanine and adenine are purine bases
42
Glucose, amino acid and fatty acid enter the citric acid cycle by their conversion into
Acetyl coA
43
A hormone which stimulates glycogenesis
Insulin
44
Chemicals extracted from organism such as bacteria and can inhibit or destroy other microorganism
Antibiotic
45
The gland or tissue that regulates the blood glucose level
Pancreas
46
Which vitamin is formed in the body by exposure to ultraviolet irradiation or sunlight
D
47
Excess vit A and D is stored in the body, but excess vit B and C is readily excreted. What property shows this?
Vit C and B are water soluble | Vit A and D are fat soluble
48
It is the entire genetic makeup of an organism
Gene
49
The vitamin which is used in the prevention of degenerative changes in the CNS
Vit B complex
50
It is a model which best explains the enzyme-substrate action
Lock and key
51
The activation of pepsinogen requres
HCl
52
DNA is primarily found in the
Nucleus/Mitochondria
53
It is the enzyme which hydrolyzes starch to dextrin and maltose
Amylase
54
A synthetic DNA is called
Recombinant DNA
55
Hydrolysis of ATP is an
Energy producing reaction
56
Characteristic of lipid
Hydrophobic
57
It is a condition that results when sugar level is below normal
Hypoglycemia
58
An example of globular protein
Albumin
59
Complementary base pairs in the DNA double helix are bonded by
H bond
60
Which nitrogen base is not found in DNA
Uracil Both DNA and RNA: adenine and guanine (purine); cytosine (pyrimidine) DNA - thymine RNA - uracil
61
An organic cofactor in an enzyme
Vitamins | Coenzyme
62
At what stage of glucose oxidation is moat of the energy produced
Aerobic stage
63
The best known building blocks of RNA and DNA are
Purines | Pyrimidines
64
It is responsible for the storage and transmission of genetic info
DNA
65
Build up of urea in the blood is called
Uremia
66
The transfer of genetic info from DNA by the formation of mRNA
Transcription
67
What is the end product of electron transport chain
Water
68
The energy producing reaction
Catabolic
69
It is the molecule that directs the activity of the cells
DNA
70
Te sugar involved in DNA
Deoxyribose
71
Sugar involved in RNA
Ribose
72
The common metabolic pathway
Krebs cycle or citric acid cycle
73
Rosenheims test is used to detect the presence of
Choline
74
Detects the presence of alpha amino acids
Ninhydrin
75
The process of producing fats from acetyl coA is called
Lipogenesis
76
``` The ff are test reagents to detect the presence of amino acids except Girgnards Xanthoproteic Millon-nasse Sakaguchi ```
Grignards
77
Amino acid test that gives a yellow color to tryptophan and tyrosine due to presence of phenyl group with which the nitric acid reacts
Xanthoproteic test
78
Amino acid test gives a deep red color to tyrosine due to preaence of hydroxyphenyl group
Millon-nasse
79
Amino acid test gives a reddish violet color at the zone of contact of two fluids with tryptophan
Hopkins-cole
80
The condition that lowers the pH of the blood due to starvation is called
Acidosis
81
Te subs responsible for the emulsion of fats is
Bile acids
82
Hubl's soln is used to ascertain degree of
Unsaturation
83
IUPAC name of acrolein
Propenal
84
The positive indication for the presence of glycerol in acrolein test
Silver mirror formed in the test tube
85
Cerebrosides are positive in the ff tests except Molisch Biuret Lassaigne's
Biuret
86
Osmic test is used to detect the presence of _____ in lipida
Prosthetic groups
87
The most sensitive chemical test to detect the presence of cholesterol
Liebermann-Burchard
88
``` The ff are phospholipids except Plasmalogen Lecithin Cephalin Choline ```
Choline is a nitrogenous base
89
A mixed triglyceride contains
Three diff fatty acids esterified with glycerol
90
The central compound found in the structure of sphingolipids
Sphingosine
91
Lipid whose specific teat is the Furter-Meyer test
Tocopherol
92
Ppt of ____ indicates the presence of phospholipids in the lipid sample
Ammonium phosphomolybdate
93
``` The ff are glycolipids except Globosides Phosphatides Gangliosides Cerebrosides ```
Phosphatides
94
Parent compound of phospholipids
Phosphatidic acid
95
A non-pentose sugar which is also positive for tollena test
Cellobiose
96
The reagent present in Molisch teat which is responsible for the dehydration reaction
Sulfuric acid
97
ID test to detect the presence of glycogen
Iodine
98
The only sugar readily forms insoluble osazone crystals
Mannose
99
Important structural material found in the exoskeletons of many lower animals
Chitin
100
Hydrolysis of osazones produce
Ozones
101
General term for a group of polysaccharides present on the primary cell wall
Pectin
102
Specific test for galactose, due to the formation of highly insol crystals
Mucic acid
103
Type of RNA which serves as template for the amino acid sequence being synthesized
mRNA
104
Positive indication for Anthrone test
Blue green color
105
Differentiating teat bet helical and linear polysaccharides
Iodine
106
The diff bet benedicts and barfoeds test reagent
pH of soln
107
Hydrolytic product of chitin
Acetylglucosamine
108
Glucose and fructose are
Geometric isomers
109
Alkaline bismuth reagent is used to detect the presence of
Reducing sugars
110
Action of dilute alkali on sugars
Tautomerization
111
The addition a pentose sugar to a base produces
Nucleoside
112
Nucleoside + phosphate group
Nucleotide
113
Central dogma concept wherein the RNA molecule is used as template for the synthesis of DNA molecule
Transcription
114
``` The ff proteins are present in egg white except Ovomucin Ovoglobulin Albumin Osseomucoid ```
Osseomucoid
115
Anaerobic glycosides occurs in the
Cytoplasm
116
Osazone test is also known as
Kowarsky test
117
Genetic defect characterized by mental retardation and cataract, since the unmetabolized sugar is toxic to the lens of the eyes
Galactosemia
118
Pyridoxine is a compound of this enzyme
Decarboxylase
119
In man, principal end product of protein metabolism is
Urea
120
Condition wherein acetone accumulates in the blood
Ketonemia
121
Glutamine is a _____ amino acid
Neutral
122
Oxidation product of ketone bodies
Carbon dioxide
123
Phosphoprotein found in egg yolk
Vitelin
124
Amino acids positive for sakaguchi reaction
Arginine
125
Histidine is negative for
Sodium nitroprusside
126
An official simple protein obtained from corn
Zein
127
Principle involved in the isolation of casein milk
Isoelectric ppt
128
Process of converting liver glycogen into blood glucose
Glycogenolysis
129
Genetic info is stored and carried in all cells by
Double stranded DNA
130
Principal site for the synthesis of urea
Liver
131
Pentose present in gum arabic
Arabinose
132
What is responsible for the transfer of genetic info
DNA
133
Only form of inorganic nitrogen which can be utilized by living cells
Ammonia
134
The 9 essential amino acids
``` Histidine Leucine Isoleucine Lysine Methionine Phenylalanine Threonine Tryptophan Arginine Valine ```
135
The chief end product of purine metabolism in man
Uric acid
136
Presence of glucose in appreciable amts in the urine
Glycosuria or glucosuria
137
``` The ff are tests for kidney efficiency except Phenylsulfophthelein test Urea clearance test Water output test Crystallization test ```
Crystallization
138
Growth hormone is also known as
Somatotropin
139
Condition wherein bile pigment is present in excess in the blood
Jaundice
140
Principal digestive constituent of the gastric juice
Pepsin
141
Condition wherein the conc of uric acid accumulates in blood reaches as high as 15mg%
Gout
142
The study of the composition and the chemical processes occurring in the living matter is
Biochemistry
143
What is wobble
Ability of certain anticodons to pair with codons that differ at the third base
144
The most impt function of HCl in the stomach is
Activation of pepsinogen
145
Transamination is
Conversion of amino acids to keto acids
146
The lipid that is converted to vit d2 upon irradiation
Ergosterol
147
The metabolic degradation of hemoglobin takes place principally in
Reticuloendothilial system
148
The amino acid that is important precursor of Hgb is
Glycine