1
Q
Toxic material definition
A
A material that releases a chemical in sufficient quantities to kill cells either directly or indirectly through inhibition of key metabolic pathways
2
Q
Key biological testing concepts
A
dose and amount important (delivered vs exposed)
uncertainty to extrapolation (animals to humans)
solubility characteristics
3
Q
3 in vitro assays
A
direct contact agar diffusion
elution
4
Q
In vitro standardised methods for comparison
A
number of cells growth phase cell type duration of exposure test sample size and SA
5
Q
Which cells are commonly used in in vitro testing? Why?
A
L929 mouse fibroblast
6
Q
Criticism of using in vitro cytotoxicity assays as primary biocompatibility screening test?
A
doesn’t use cells with significant metabolic activities
7
Q
In vivo tests
A
sensitisation irritation systemic and subacute toxicity genotoxicity implantation hemocompatibility chronic toxicity carcinogenicity immune response
