Bioreactor Study Guide

Question 1

Explain flow perfusion bioreactors.

Answer 1

• More homogeneous cell distribution as compared to static bioreactor
• Best for fluid transport
• Fluid pump is used to force fluid through the scaffold
• Media can be easily replaced in media reservoir

Question 2

Name the basic types of bioreactors

Answer 2

1. spinner flask
2. rotating-wall vessel
3. Hollow fiber
4. direct perfusion
5. load cell

Question 3

Define bioreactor.

Answer 3

a system in which conditions are closely controlled to permit or induce certain behavior in living cells or tissues

Question 4

What is a Spinner Flask Bioreactor?

Answer 4

• Basic bioreactor
• Scaffold suspended at end of needles
• Magnetic stirrer mixes media, while scaffold remain fixed
• Typically smaller volumes • medium changed every 2 days

Question 5

Why do we need to use a bioreactor

Answer 5

• precondition tissue, many tissues are in motion when in the body
• trigger natural stimulation in vitro, by patterning in vivo mechanical stimulation, we can alter expression profiles

Question 6

When would a compression bioreactor be used?

Answer 6

used in cartilage engineering

Question 7

How can we create accurate flow systems in a bioreactor?

Answer 7

by modeling of physiology using fluid dynamics

Question 8

How does fluid flow help cells grow?

Answer 8

1. it can simulate shear stress to stimulate proper growth
2. it can provide nutrients in and remove waste
3. can assist in a better efficient even layer for cell seeding and growth. more uniformly distributed cells.

Question 9

What are the advantages of preconditioning tissue

Answer 9

since most tissues are in motion when they are in the body, preconditioning the tissue will allow for the tissue to grow properly to maintain the strength and adequate function it will need later…..

this can be done by patterning in vivo mechanical stimulation, we can alter expression profiles

Question 10

What different kinds of stimulus can you apply using bioreactors?

Answer 10

compression- through pistons and weiights

strain- bending and stretching

shear- fluid flow, spinning (rotating)

Question 11

. Name the five different basic types of bioreactors.

Answer 11

1. spinner flask
2. rotating-wall vessel
3. Hollow fiber
4. direct perfusion
5. load cell

Question 12

What are three different methods of applying stimuli to a bioreactor?

Answer 12

compression- through pistons and weiights

strain- bending and stretching

shear- fluid flow, spinning (rotating)