Conjugation
Direct cell-to-cell contact for DNA transfer
What are Transposons?
They are also called Jumping genes.
It is a DNA sequence thar can change within a genome and can alter a cell’s genetic identity and genome size
Transduction
Relies of bacteriophage for transfer of DNA between cells
Transformation
Uptake of free or naked DNA from the environment
What is a Vector?
It is an extra chromosomal genetic material capable of SELF-REPLICATION
What are the ideal characteristics of a VECTOR?
- It should have an Origin or Replication or ORI region (in other words, it should be self-replicating
- It should contain 1 Restricting site bt different restricting enzymes
- It should have a Marker gene (to help identify the recombinant DNA)
- It should have a Transfer Gene (allows the gene to transfer itself into the host cell)
Examples of vectors
Plasmid, Bacteriophage
Which instrument is used for PCR?
Thermocycler
What is the storage condition for Whole Human Blood?
It should be stored in Cold condition
Shelf life - 21 days
Disadvantage - Volume overload
What is the shelf life of Platelets?
5 days
Name the 3 types of Plasma components.
Fresh frozen plasma
Cryoprecipitate
Dry Human Plasma
What is the storage condition of Fresh Frozen Plasma?
-30°C
It has a shelf life of 1 year
What is the storage condition of Cryprecipitate?
-30°C and Shelf life of 1 year
IT SHOULD BE THAWED AT 30-37°C
What is the technique used for Protein separation in fractionalization of Plasma?
Cohn’s technique
What is Cryoprecipitate?
It is a blood product made from fresh frozen plasma, containing a concentrate of clotting factors and other proteins
What is Western, Southern and Northern Blotting technique used for?
Western - Protein
Southern - DNA
Northern - RNA
