Biotechnology

Question 1

Define restriction endonuclease and who found them?
Aka molecular scissors

Answer 1

They restrict the growth of virus/ bacteriophage in bacteria.
Isolated in 1963 by arber nathans and smith

Question 2

Seq of ECOR1

Answer 2

5’ GAATTC 3’

Staggered

Question 3

More than 900 restriction enzymes have been isolated from how many different strains of bacteria?

Answer 3

230

Question 4

First restriction enzyme to be isolated was?

Answer 4

HIND 2 (II)

5’GTTACC3’

haemophillus influenza

Blunt ends

Question 5

First recombinant plasmid?

Answer 5

Stanley Cohen and Herbert Boyer in 1972

Native plasmid of salmonella typhimurium was inserted into E Coli ( resistance to streptomycin)

Question 6

Father of genetic engineering

Answer 6

Paul berg

SV-40 ( simian virus ka gene was inserted into E Coli with the help of bacteriophage lambda

Question 7

On hydrolysis of x-gal (substrate) blue colour is seen in which?

Answer 7

Transfomant non recombinant

Question 8

Agrobacterium tumefacians ke baare mein batao

Answer 8

Causes crown gall disease in dicots. It is a gram negative bacteria
It has Ti (tumor inducing) plasmid.

Question 9

In Ti plasmid.. which region is essential for transfer and integration of T- dna ( transfer dna) into plant cells?

Answer 9

Vir region / virulence region.

Question 10

Wat are retro viruses?

Answer 10

Used to infect animal cells

Can cause cancer

Question 11

Shuttle vectors?

Answer 11

Have 2 ori sites -
One for eukaryotes and one for prokaryotes

Eg- yeast episomal plasmid (YEp)

Question 12

Why is chilled ethanol used in isolation of dna

Answer 12

Jab chilled ethanol ko daalenge to dna precipitates out and via spooling ham use remove kr skte

Question 13

Dna fragments in agrose gel media move towards?

Answer 13

Anode (+)

Question 14

Wat is pcr?

Answer 14

Dna replication on a large scale!

Pcr- polymerase chain reaction

Question 15

Wat is a competent host?

Answer 15

Host which has the ability to pick up the r-dna

Question 16

Golden rice is enriched in?

Answer 16

Vitamin A

Question 17

Protein coded bt cry 1 Ab controls?

Answer 17

Corn borer

Question 18

Protein coded by cry 1 Ac and cry 2 Ab control?

Answer 18

Cotton bollworms

Question 19

Examples of lepidopterans

Answer 19

Tobacco budworm
Armyworm

Question 20

Coelpterons

Answer 20

Beetles

Question 21

Dipeterans?

Answer 21

Flies and mosquito

Question 22

The bt toxin is coded by?

Answer 22

Cry gene.. cry 1 Ac

Question 23

Wat is Meloidegyne incognitia?

Answer 23

A nematode which infects the roots of tobacco plants

Question 24

State t/f

RNAi takes place in some specific eukaryotes as a method of physiological defence

Answer 24

FALSE..

it takes place in all eukaryotes as a method of cellular defence

Question 25

Which vectors were used to introduce nematode specific genes into host plant?

Answer 25

Agrobacterium vectors

Question 26

In mammals including humans insulin is synthesized as

Answer 26

Pro hormone.. ie it needs to be processed before it becomes fully functional

Question 27

Wat is not present in the mature insulin?

Answer 27

C-peptide is not present in the mature insulin.

Question 28

Wat was the main challenge for production of insulin using rdna technique?

Answer 28

Getting insulin assembled into a mature form.

Question 29

Which enzyme is crucial for the immune system to work?

Answer 29

ADA( adenosine deaminase)

Question 30

Wat are the conventional methods of diagnosis?

Answer 30

Serum and urine analysis

Question 31

ELISA is based on which principle?

Answer 31

Antigen-antibody interaction ELISA- Enzyme Linked Immuno-sorbent Assay

Question 32

Which sequence is responsible for controlling the copy number of the linked dna

Answer 32

Ori

Question 33

Dna is hydrophilic molecule true / false

Answer 33

TRUE

Question 34

Wat helps in identifying non transformants

Answer 34

Selectable markers

Question 35

A procedure through which a piece of dna is introduced in a host bacterium

Answer 35

Transformation

Question 36

Useful selectable marker for E COLI are?

Answer 36

Genes encoding resistance to antibiotics such as Ampicillin Chloramphenicol Tetracycline Kanamycin

Question 37

ELISA is not an application of pcr.. wat all arre its application???

Answer 37

Diagnosis of pathogens Paleontology Diagnosis of sickle cell anemia