blood components

Question 1

blood

Answer 1

unique connective tissue
- has fibers and fibrgenin for clotting

Question 2

blood smear

Answer 2

1. acidic dye - eosin
2. basic dye - methyl blue
3. azure dyes - stain azurophilic granules (lysosomes) light purple

Question 3

common mixtures for blood smears

Answer 3

wright’s stain
giemsa stain

Question 4

fibers and proteins in blood

Answer 4

• albumin
• globulins
• lipoproteins
• hormones

Question 5

platelets

Answer 5

not cells, fragments of megakaryocyte

Question 6

serum

Answer 6

plasma without clotting factor

Question 7

blood hematocrit

Answer 7

• plasma ~55%
• buffy coat
  1. leukocytes (WBCs) - 1%
• neutrophils - 60-70%
• lymphocytes - 20-25%
• monocytes - 3-8%
• eosinophils - 2-4%
• basophils - .5-1
  2. platelets <1%
• hematocrit (RBCs) ~45%

Question 8

blood plasma

Answer 8

1. water 90-92%
2. 7-8%
   - albumin, globulins, fibrinogen
3. small molecules 1-2%

Question 9

albumin

Answer 9

produced in the liver
- maintains osmotic pressure
- acts as carrier protein

Question 10

glubulins

Answer 10

10

Question 11

fibronegin

Answer 11

10

Question 12

erythroid precursor cells

Answer 12

reticulocyte
- extrudes the nucleus

LESS NUCLEUS AND RNA
ORGANELLE FREE
MORE HEMOGLOBIN AND CYTOPLASM

Question 13

RBC

Answer 13

2.6 microns at widest
.8 microns at skinniest

• central pallor (paleness)

Question 14

spectrin

Answer 14

structural protein that maintains shape

Question 15

erythrocyte abnormalities

Answer 15

1. hemolytic anemias
2. abnormal Hb, less viable RBCs
   - sickle cell
3. defects in membrane/cytoskeletal anchoring proteins
   - hereditary spherocytosis
4. immune attack on RBCs
   - immunohemolytic anemias

Question 16

leukocytes

Answer 16

• lobes
• granules
• lysosomes
• binds dyes (azurophilic)

Question 17

agranulocytes

Answer 17

• monocytes
• lymphocytes