CB3bii

Question 1

Describe the method used to extract DNA from fruit

Answer 1

Place a piece of fruit in a beaker and crush it
Add detergent and salt mix
Filter the mixture and collect the liquid in a test tube
Pour chilled ethanol into the test tube
DNA precipitates forming a fibrous white solid
Use a glass rod to collect the DNA sample

Question 2

Why is detergent added to the crushed fruit

Answer 2

It disrupts the cell membranes, releasing DNA into solution

Question 3

Why is salt added to the crushed fruit

Answer 3

Salt encourages the precipitation of DNA