CB4 Genetic Engineering (Higher Only)

Question 1

What 2 enzymes are used in genetic engineering?

Answer 1

Restriction, ligase

Question 2

What 2 vectors can be used in genetic engineering?

Answer 2

Bacterial plasmid or virus

Question 3

What 2 steps are restriction enzymes used for in genetic engineering?

Answer 3

Cutting the desired gene out of the genome, cutting open the bacterial plasmid

Question 4

What is a DNA “sticky end”?

Answer 4

A staggered cut of DNA, leaving exposed, unpaired bases.

Question 5

What step is ligase enzyme used for in genetic engineering?

Answer 5

Attaching the desired gene to the bacterial plasmid