Ch 11 Preforming Common Technical Procedures

Question 1

When giving an IP (intraperitoneal) injection, what 2 anatomical structures do you attempt to avoid by injecting into the lower right quadrant of the abdomen?

Answer 1

The bladder and cecum

Question 2

What 2 anatomical structures should be avoided when giving an IM (intermuscular) injection into the quadriceps?

Answer 2

Sciatic nerve and the femoral vein

Question 3

When administering an IC (intracranial) injection, what 3 anatomical landmarks should be identified?

Answer 3

1. Sagittal suture
2. Bregma
3. Lambda

Question 4

What is the MAX volume that can be injected into an adult mouse RO (retro-orbitally)?

Answer 4

150 microliters

Question 5

What is slightly withdrawing the plunger of syringe after placement of the of the needle is called what?

Answer 5

Aspiration

Question 6

Which of the following is an accurate list of routes of injection from fastest absorption (left) to slowest (right)?

Answer 6

IV-IP-IM-SC-ID

Question 7

Which type of injection has a risk of puncturing the bladder?

Answer 7

Intraperitoneal

Question 8

What is the common guideline for the safe percentage of blood volume, expressed as a percent of the body weight, that can be taken every 2 weeks?

Answer 8

1%

Question 9

Which animal is NOT routinely bled from the jugular vein?

Answer 9

Hamsters

Question 10

In which animal, other than the rabbit, is arterial blood frequently collected via the auricular artery?

Answer 10

Pig

Question 11

Which administration technique is commonly used in aquatic species?

Answer 11

Intracoelomic injection

Question 12

Where are Xenopus typically given an IM injection?

Answer 12

Quadriceps muscles

Question 13

From which location can serial blood samples be obtained from zebrafish?

Answer 13

Dorsal aorta

Question 14

For larger fish, from which location could repeated blood collections be efficiently taken?

Answer 14

Caudal vein

Question 15

To collect sperm from a male zebrafish, what is avoided by ensuring that the urogenital pore is dry?

Answer 15

Activation of the sperm

Question 16

Where should female Xenopus be injected with hormones to prime them for egg harvest?

Answer 16

Dorsal lymph sacs

Question 17

Which testing device uses pads containing different chemical reagents to test urine for various parameters?

Answer 17

Dipstick

Question 18

An animal’s total blood volume is approximately what percentage of its body weight?

Answer 18

6%

Question 19

A vein should be punctured at an angle of approximately _____ degrees, with the bevel facing away from the skin.

Answer 19

30

Question 20

It is recommended that neonatal mice receive retroorbital injections no greater than what volume?

Answer 20

10 microliters

Question 21

It is recommended that adult mice receive retroorbital injections no greater than what volume?

Answer 21

150 microliters

Question 22

What indicates a correct intradermal injection?

Answer 22

A bleb at the site

Question 23

From which location can serial blood samples be obtained from zebrafish?

Answer 23

Dorsal aorta

Question 24

Which device can be used to collect blood?

Answer 24

Microhematocrit tube

Question 25

Which is NOT a method of obtaining sperm from Xenopus species?

Answer 25

Cloacal lavage

Question 26

Which type of injection has a risk of puncturing the bladder?

Answer 26

Intraperitoneal

Question 27

Where are Xenopus typically given an IM injection?

Answer 27

Quadriceps muscles

Question 28

Which is NOT a method of obtaining sperm from Xenopus species?

Answer 28

Injection of human chorionic gonadotropin (hCG) hormone

Question 29

Hemolysis is?

Answer 29

a breaking apart of the blood cells

Question 30

Where is the saphenous vein located?

Answer 30

on the lateral side of the lower rear leg

Question 31

To collect blood by cardiac puncture, entry into the heart can be made through the ___________ space (between the ribs)

Answer 31

intercostal

Question 32

What is cystocentesis?

Answer 32

Drawing of sterile urine by percutaneously puncturing the bladder

Question 33

What is the easiest method for sampling blood form Xenopus?

Answer 33

Cardiocentesis (cardiac puncture)

Question 34

Which administration technique is commonly used in aquatic species?

Answer 34

Intracoelomic injection

Question 35

Which vein is most commonly used for intravenous injections in the rat?

Answer 35

Lateral tail vein

Question 36

What is the maximum number of surgeries for oocyte collection recommended over the life of a Xenopus?

Answer 36

6