Ch. 20 Genomics

Question 1

structural genomics

Answer 1

concerned with the organization and sequence information in a genome

Question 2

how do you make a genomic library?

Answer 2

1. different DNA molecules are cut in different places providing a set of overlapping fragments
2. each fragment is joined to a cloning vector, transferred to bacterial cells
3. this produces a set of clones containing overlapping genomic fragments (some contain gene of interest)
4. bacteria can be spotted and screened with a probe to identify the candidate clones (like a southern)

Question 3

linkage analysis

Answer 3

-looking at 2 different features of the genome that occur together because of their proximity to each other
(recombination frequency)

Question 4

recombination frequency

Answer 4

equals recombinant gametes over total gametes
= recombinant progeny/total progeny
-measured in centimorgans (cM)

Question 5

positional cloning

Answer 5

allows us to connect a gene of interest with a previously mapped linked gene (or marker)
(“chromosome walking”)

Question 6

southern and northern blots

Answer 6

to detect the presence/absence of specific DNA sequences