Why is our knowledge of microbial diversity so limited?
small, hard to culture, need special nutrients, and part of complex food chains requiring products of other bacteria
Dichotomous key(DK) for prokaryotes step 1 first split
Prokayotic ->
DK- Pseudomurein rRNA signiture leads to
Domain Archaea (end of line on DK)
DK- Peptidoglycan rRNA signiture leads to
Domain Bacteria (splits for next step)
DK- Domain Bacteria (second split)
2. Gram-positive
DK Gram-negative split
2. Nonproteobacteria
DK Gram-positive split
2. High G+C
extremeophiles
Domain Archaea
-hyperthermophiles, methanogens, and extreme halophiles