CHAPTER 2

Question 1

Recommended for aqueous solutions, such as buffers, diluted acids, or alkalis.

Answer 1

Forward Technique

Question 2

This technique is commonly used when pipetting and mixing a sample or reagent into another liquid.

Answer 2

Forward Technique

Question 3

1. Press the operating button to the first stop.
2. Dip the tip into the solution to a depth of 1 cm, and slowly release the operating button. Withdraw the tip from the liquid, touching it against the edge of the reservoir to remove excess liquid.
3. Dispense the liquid into the receiving vessel by gently pressing the operating button to the first stop. After one second, press the operating button to the second stop. This action will empty the tip. Remove the tip from the vessel, sliding it along the wall of the vessel.
4. Release the operating button to the ready position

Answer 3

Forward Technique

Question 4

This is for standard pipetting

Answer 4

Forward Technique

Question 5

This is for solutions with high viscosity or tendency to foam

Answer 5

Reverse Technique

Question 6

This technique is commonly used with air displacement pipettes

Answer 6

Reverse Technique

Question 7

This is recommended for precisely pipetting small volumes.

Answer 7

Reverse Technique

Question 8

This avoids the risk of sample splash, foaming, or bubble formation.

Answer 8

Reverse Technique

Question 9

1. Press the operating button to the second stop.
2. Dip the tip into the solution to a depth of 1 cm, and slowly release the operating button. This action will fill the tip. Withdraw the tip from the liquid, touching it against the edge of the reservoir to remove excess liquid.
3. Dispense the liquid into the receiving vessel by depressing the operating button gently and steadily to the first stop. Hold the button in this position. Some liquid will remain in the tip, and this should not be dispensed. Remove the tip by sliding it along the wall of the vessel.
4. The liquid remaining in the tip can be pipetted back into the original solution or thrown away with the tip.
5. Release the operating button to the ready position.

Answer 9

Reverse Technique

Question 10

This is for repeat pipetting of the same volume

Answer 10

Repetitive Pipetting Technique

Question 11

This technique is ideal for adding reagents into tubes or wells of microplates

Answer 11

Repetitive Pipetting Technique

Question 12

1. Press the operating button to the second stop.
2. Dip the tip into the solution to a depth of 1 cm, and slowly release the operating button. Withdraw the tip from the liquid, touching it against the edge of the reservoir to remove excess liquid.
3. Dispense the liquid into the receiving vessel by gently pressing the operating button to the first stop. Hold the button in this position. Some liquid will remain in the tip, and this should not be dispensed. Remove the tip by sliding it along the wall of the vessel.
4. Continue pipetting by repeating steps 2 and 3.

Answer 12

Repetitive Pipetting Technique

Question 13

This technique is used for pipetting heterogeneous samples, such as blood or serum.

Answer 13

Heterogeneous Sample Pipetting Techniqu

Question 14

Pre-rinsing the tip is not possible, and the full sample should be dispensed for accurate analysis.

Answer 14

Heterogeneous Sample Pipetting Techniqu

Answer 15

Heterogeneous Sample Pipetting Techniqu