1
Q
Microbial cells are enumerated by what?
Are they reliable?
(Definition of enumerated - to ascertain the number of : count)
A
Microscopic Observations
No
2
Q
What are the limitations of microscopic counts?
Hint: There are a lot
A
- Cannot distinguish between live and dead cells without special stains
- Small cells can be overlooked
- Precision is difficult to achieve
- Phase-contrast microscope required if a stain is not used
- Cell suspensions of low density (<106 cells/ml) hard to count
- Motile cells need to immobilized
- Debris in sample can be mistaken for cells
3
Q
What is a second method for enumerating (counting) cells in a liquid sample?
A
Using a flow cytometer
A Flow Cytometer uses lasers, fluorescent dyes, and electronics
Micro Test 1
flashcards
Decks in class (21)
# Cards
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Chapter 5.9 Viable Counts7
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Chapter 5.3 MreB and Cell Morphology7
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Chapter 5.4 Peptidoglycan Biosynthesis8
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Chapter 5.5 Quantitative Aspects of Microbial Growth7
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Chapter 5.6 The Growth Cycle6
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Chapter 5.7 Continuous Culture6
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Chapter 5.8 Microscopic Counts3
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Chapter 5.1 and 5.2 Binary Fission and Fts Proteins and Cell Division10
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Chapter 4.1 Macromolecules and Genes9
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Chapter 4.2 The Double Helix11
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Chapter 4.3 Genetic Elements: Chromosomes and Plasmids10
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Chapter 4.4 Templates and Enzymes6
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Chapter 4.5 The Replication Fork5
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Chapter 4.6 Bidirectional Replication and the Replisome8
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Chapter 4.7 Transcription17
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Chapter 4.8 and 4.9 The Unit of Transcription and Transcription in Archaea and Eukarya14
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Chapter 4.10 Polypeptide, Amino Acids, and the Peptide Bond6
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Chapter 4.11 Translation of the Genetic Code12
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Chapter 4.12 Transfer RNA4
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Chapter 4.13 Protein Synthesis10
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Chapter 4.14 Protein Folding and Secretion13
