Codon Recognition By tRNA

Question 1

Describe antiparallel binding between codon and anti codon

Answer 1

• binding follows rules of complimentary base pairs

- 5’-3’ codon read and paired by 3’-5’ anticodon

Question 2

Describe wobble hypothesis and application

Answer 2

• 1st 2 nucleotides on anticodon follow Watson-crick rules
• last nucleotides of anti codon not spatially defined / less stringent

-allow a tRNA to recognize more than 1 codon

Question 3

List the purines and pyrimidines

Answer 3

1 Adenine / guanine

2 cytosine/ uracil / thymine

Question 4

List the wobble base pairs

Answer 4

1st is tRNA and 2nd mRNA

A - U
C - G
G - C / U
U - A / G
H - U / C / A

Question 5

Describe hypoxanthine

Answer 5

-product of adenine deamination and base in e nucleotide inosine monophosphate