COMPONENT PREPARATION AND INDICATION

Question 1

It is up to 500cc but in the bag is up to 450 cc only

Answer 1

> FRESH WHOLE BLOOD

Question 2

How many cc that can contain in tubing fresh whole blood?

Answer 2

> 30-50cc blood; 450cc for the main bag

Question 3

Subjected to ____________ = PLATELET RICH PLASMA will separate from packed RBC

Answer 3

> Soft or light spin centrifugation

Question 4

________ packed RBC for fresh whole blood

Answer 4

> Lower

Question 5

What is the step by step process of Tubing?

Answer 5

• Break the seal of the main bag and with seal packer it is
  manually done
• The plasma will be transferred to the transfer bag,
  retain small portion of plasma in the packed RBC
• Plasma will served as nutrients or food (glucose) in the cells.

Question 6

Subjected to hard spin the platelets settled at the
bottom or lower layer of plasma

Answer 6

> PLATELET RICH PLASMA

Question 7

Platelet that has poor plasma for platelet rick plasma.

Answer 7

> UPPER LAYER

Question 8

Platelet that concentrate in platelet rich plasma.

Answer 8

> Lower layer

Question 9

What will happen if you break the sealed of the 1st transfer bag in platelet rich plasma?

Answer 9

> Allowing the platelet poor plasma to transfer to another bag

Question 10

How many cc of plasma transfer which is platelet concentrate?

Answer 10

> 50-70cc only

Question 11

In the platelet rich plasma it sealed both side ____________

Answer 11

> At the middle cut to separate 2 transfer bag

Question 12

Platelet concentrate placed at room temperature allow to sit or stand undisturbed for ___________.

Answer 12

1. 1 hour

Question 13

How long should the substance be left at room temperature in the agitator machine, and what purpose does this serve?

Answer 13

1. 3 to 5 days at room temperature in agitator machine
2. Prevent for adhering to one another “platelet aggregation”

Question 14

What is the procedure for handling Platelet Poor Plasma (PPP) and its comparison to Fresh Frozen Plasma (FFP)?

Answer 14

• Platelet Poor Plasma (PPP) can be directly frozen like Fresh Frozen Plasma (FFP).
• Unlike PPP, FFP contains all the coagulation factors necessary for the patient, including those from the intrinsic, extrinsic, and common pathways.

Question 15

What is the procedure for preparing FRESH FROZEN PLASMA, and what equipment is used?

Answer 15

> FRESH FROZEN PLASMA is prepared using the Multiple Transfer Bag procedure

Question 16

What is the process for transforming fresh frozen plasma transferred at a refrigerated temperature into liquid plasma?

Answer 16

> The process involves undergoing a slow cold thawing process, allowing the fresh frozen plasma to transform from its frozen state to liquid plasma.

Question 17

What occurs after the thawing process with regards to the separation of components?

Answer 17

• The lower component undergoes precipitation to form cryoprecipitate, while the upper component, known as plasma or clear liquid, is referred to as cryosupernate.

Question 18

What is the composition of CRYOSUPERNATE and how does it compare to Fresh Frozen Plasma (FFP) in terms of coagulation factors?

Answer 18

• Same coagulation factors found in FFP, but some of these factors have decreased due to precipitation.
• This results in a higher concentration of these coagulation factors in the cryoprecipitate layer of CRYOSUPERNATE.

Question 19

Enumerate the 5 components of CRYOSUPERNATE

Answer 19

1. Factor 8 C
2. Factor 1 or fibrinogen
3. Factor 13
4. Von willie brand factor
5. Fibronectin

Question 20

What components is cryoprecipitate rich in?

Answer 20

• Factor 1, Factor 8 c, Von willie brand, Factor 13 or fibrin clot stabilizing factor, Fibronectin