Connective Tissue Lab

Question 1

Starting W/ Special C.T. pg. 30 BLD.

Answer 1

Place keeper card.

Question 3

Cell Type?

Answer 3

RBC’s

Question 4

Cell Type?

Answer 4

Reticulocyte (Immature RBC.)
Notice: Nuclear Extrusion, larger, more Basophilic (Blue).

Basophilic: Attraction to Basic/ Cationic Dye- H&E: Haematoxylin.

Question 5

Cell Type?

Answer 5

Reticulocyte (Immature RBC.)
Notice: Anucleate, larger, more Basophilic (Blue).

Basophilic: Attraction to Basic/ Cationic Dye- H&E: Haematoxylin.

Question 6

What are the 4 ways of recognizing Reticulocytes?
Specific Stain you can use for recognition?

Answer 6

• Somewhat Basophilic Cytoplasm than RBC.
• Nuclear Fragments or Anucleate.
• Larger.
• Less uniform (Still Biconcave).
• New Methylene Blue Stain: Reticular/ Granular Network (Absent in Mature.)

Basophilic: Attraction to Basic/ Cationic Dye- H&E: Haematoxylin.
Reticulocyte: Immature RBC.
Nuclear Extrusion/ Enucleation: Nuclear Expulsion & Breakdown.

Question 7

What is the Development of an RBC?

Answer 7

1. Mesenchyme.
2. Hemocytoblast.
3. Myeloid Progenitor.
4. Proerythroblast/ Pronormoblast.
5. Basophilic Erythroblast.
6. Polychromatic Erythroblast.
7. Orthochromatic Erythroblast/ Normoblast.
8. Polychromatic Erythrocyte/ Reticulocyte.
9. Erythrocyte (Complete/ Mature.)

Question 8

What does Orthochromatic mean?
In Relation to RBC’s?

Answer 8

Orth/o: Normal.
Chrom/o: Color.
Erythrocyte: Refers to the precursor cell (Polychromatic Erythroblast) becoming more eosinophillic/ normal Colored due to filling with Hemoglobin @ this Stage.
Still more Basophiliic than next step: Polychromatophilic Erythrocyte/ Reticulocyte.

Question 9

What are the 5 types of Leukocytes?

Answer 9

• Basophils (Granular).
• Neutrophils (Granular).
• Eosinophils (Granular).
• Lymphocytes (Agranular).
• Monocytes (Agranular).

Granular Vs Agranular: Visible Granules.