CP2 Microscopy

Question 1

What are the 3 skills tested in this practical?

Answer 1

• Using compound light microscope safely.
• Measuring actual size of cells.
• Drawing cells.

Question 2

How should you carry the microscope to your bench?

Answer 2

• Using both hands.

Question 3

What are the different lenses on a microscope called?

Answer 3

• Objective lenses.

Question 4

How do you measure the actual size of cells?

Answer 4

• Measure the cell using eyepiece graticule.
• Calibrate graticule scale by alignment of graticule + stage micrometer scales.

Question 5

How do you do a biological drawing of a cell?

Answer 5

• Line drawings - no sketchy lines.
• Labels.
• Only draw what you aee.

Question 6

What is the function of a stage micrometer?

Answer 6

• Calibrate eyepiece graticule to use for measurements.

Question 7

How to prepare a slide?

Answer 7

• Stain sample w/ dye.
• Mount sample on slide.
• Apply coverslip avoiding air bubbles.

Question 8

What is the formula for magnification?

Answer 8

• M=I/A
• Magnification = image / actual.

Question 9

What are the requirements for label lines in biological drawings?

Answer 9

• Horizontal.
• Straight.
• Not crossing other lines.

Question 10

Outline procedures of focusing w/ light microscope.

Answer 10

• Rotate nosepiece to lowest objective lens.
• Look through eyepiece and use coarse focus to focus image.
• Use fine focus knob to fine-tune image.