CP5-Beetroot Membrane

Question 1

Objectives- investigate the effect of temperature on membrane permeability

Answer 1

Objectives:

-know how to effect of temperature on membranes can be determined

Question 2

Risks?

Answer 2

• water baths/Bunsen burners can burn you
• cork borer and knives are sharp
• be careful around neat sources, remove anything flammable, keep on safety flame when not in use
• be careful when using sharp items and cut away from self

Question 3

Method?

Answer 3

• prepare 8 water baths with temperatures ranging between 0 and 70 degrees, at 10 degree intervals, using a thermometer
• use a syringe to add 10 cm^3 of distilled water to 8 test tubes and label with temperatures from the range of water baths
• place each tube in a water bath for 10 minutes
• cut 8 pieces of beetroot using the same cork borer and cut to the same length of 1cm long and wash the cylinders until they run clear and dry gently with a paper towel
• add one beetroot to each water bath and leave for 15 minutes
• set the colorimeter to 0 by using a cuvette of distilled water as a reference value
• pour 2cm^3 of the supernatant from each water bath into a cuvette and record the absorbance of the solution without the beetroot piece
• record in a table and plot a graph of transmission against temperature

Question 4

Variables?

Answer 4

IV-the temperature of the water bath
DV-the absorbance of the supernatant
CV- size and mass of beetroot, type of beetroot, same pre-treatment