Specific esterase
What is it positive in
Positive in myeloid cells
In AMML myeloblasts are red with specific
Non specific esterase
Positive in monocytes
In AMML, monoblasts are blue with nonspecific
Periodic Acid Schiff (PAS)
What is positive, what is negative?
Positive in lymphoblasts (ALL) and erythroblasts (AEL, DiGuglielmo’s)
Normal erythroblasts will be negative
Leukocyte Alkaline Phosphatase (LAP)
Positive in leukemoid reactions
NOT in neoplasms, NOT in CML
Tartrate Resistant Acid Phosphatase (TRAP)
Positive in hairy cells
Terminal Deoxynucleotidal Transferase (TdT)
Method of interpretation?
Positive in most acute lymphocytic leukemia ALL
Interpreted with immunofluorescence