What is decalcification?
The process of removing calcium salts from tissue before further tissue processing
What are the advantages of microwave decalcification?
Fast, economical, reliable, provided the end point is not exceeded
What happens if the decalcification end point is exceeded?
Causes a decrease in staining, most obvious in the nucleic acids
How long do traditional decalcification methods typically take?
Days or weeks to complete, depending on the density of the bone
What is the benefit of microwave decalcification compared to traditional methods?
Reduces the time significantly, providing better turnaround time of the specimen
What should be done to specimens before decalcification?
Specimens should be thoroughly fixed to prevent soft tissue damage
What is the rinsing requirement after fixation and before decalcification?
Rinse the specimens with water
What is the fluid volume requirement during the decalcification process?
Approximately 100 times the volume of the tissue
What is the first step in marrow decalcification for specimens less than 3mm?
Zinc Formalin at 40°C for 30 minutes
What is the second step in marrow decalcification for specimens less than 3mm?
Wash in water
What is the third step in marrow decalcification for specimens less than 3mm?
Decal (Biocare) at 40°C for 30 minutes
What is the first step in bone marrow decalcification for specimens 3-5mm?
Zinc Formalin at 40°C for 60 minutes
What is the second step in bone marrow decalcification for specimens 3-5mm?
Wash in water
What is the third step in bone marrow decalcification for specimens 3-5mm?
Decal (Biocare) at 40°C for 30 minutes
What may affect the decalcification time for bone marrow specimens?
Sample thickness or bone density; it may take up to 2 hours
