Deconstructing the Genome

Question 1

Cloning DNA steps

Answer 1

1 - ligation of fragments to cloning vectors creates recombinant DNA molecules
2 - Host cells take up and amplify vector-insert recombinants

Question 2

cloning vectors

Answer 2

allow us to select amplify and maintain specific gene fragments in bacteria

Question 3

common vectors

Answer 3

plasmids
phages
bacterial and yeast artificial chromosomes

Question 4

plasmids contain

Answer 4

origin of replication
selectable marker
restriction sites

Question 5

libraries

Answer 5

just a collection of cloned fragments