Dehydrogenase Activity

Question 1

Describe DCPIP

Answer 1

A blue dye that acts as an electron acceptor
Reduction causes it to decolourise
If placed in isolated chloroplasts it will accept electrons from the electron transport chain and hence be reduced

Question 2

Give three independent variables that could be tested in this experiment

Answer 2

Light intensity - move lamp closer/ further
Light wavelength - place different filters over the lamp
Concentration of weedkiller - using ammonium hydroxide to mimic

Question 3

Give the control variables that must be mainatined in this experiment

Answer 3

Source of chloroplasts
Volume of chloroplast suspension
Volume of DCPIP
concentration of DCPIP