why is dermal puncture the preferred blood collection technique on infants and v small children?
who else can dermal puncture be good for?
Children
Infants
Elderly patients
Oncology patients
Severely burned patients
Obese patients
Inaccessible veins
Extremely fragile veins
Home testing by patient
Point-of-care testing
Procedure requiring capillary
specimen only
Patients with thrombotic
(clotting) tendencies
what tests can not be done with dermal puncutre
coagulation tests, blood culture and ESR (last 2 need lots of blood)
what is the specimen botained during dermal capillary puncture?
mixture of venous blood, arterial blood, and interstitial fluid (fluid between
cells and tissues). When capillary blood is drawn it must be noted for the testing
personnel. Capillary blood is higher in glucose and lower in potassium, total
protein, and calcium making the normal values (reference ranges) different
how to select site for dermal puncture?
choosing heel spot for dermal puncture?
equipment for dermal puncture
● Requisition slip
● Gloves
● Alcohol prep pad
● Gauze
● Adhesive bandage or tape
● Sharps container
● Computer label
● Permanent marker or pen
● Safety dermal (capillary) puncture device or lancet
● Microspecimen containers
regions to do dermal puncture
Heel; medial and lateral plantar surfaces
Central fleshy area of third and fourth fingers Across fingerp`rint lines
areas not to do dermal puncture
Callused finger (usually index finger), thumb, or pinkie
Back of heel, bottom of foot, arch of foot
Along fingerprint lines
Areas with visible damage or edema
Sites previously used for dermal (capillary) puncture
what is diff about the actual dept hof the puncture and the lancet
the actual depth of puncture is slighlt more than the lancet length because the pressure punctures the skin
- devices r usually set at 2.2 mm for children over 8 and adults
depth of puncture depth for premature and full term neonates
premature - 0.85 mm
full term - less than 2 mm
depth of puncutre for child 6 months to 8 years
1.5 mm
depth of punctre for child over 8 and adults
2.4 mm
how to preform dermal puncture
how to immobilize pediatric pt for dermal puncture?
how to collect dermal specimen
what is the order to draw for dermal puncture
● EDTA (lavender or pink)
● Heparin (green or light green)
● Sodium fluoride (gray)
● Nonadditive (red) or serum separator (gold)
complicatiosn of dermal puncture
Maximum Allowable Volume
(mL) in One Blood Draw (2.5% of
Total Blood Volume)
Total Maximum Allowable Volume
(mL) Drawn Over 30 Days for 1 kg
2.5 and 5
Maximum Allowable Volume
(mL) in One Blood Draw (2.5% of
Total Blood Volume)
Total Maximum Allowable Volume
(mL) Drawn Over 30 Days for 2 kg
5 and 10
Maximum Allowable Volume
(mL) in One Blood Draw (2.5% of
Total Blood Volume)
Total Maximum Allowable Volume
(mL) Drawn Over 30 Days for 3` kg
6 and 12
Maximum Allowable Volume
(mL) in One Blood Draw (2.5% of
Total Blood Volume)
Total Maximum Allowable Volume
(mL) Drawn Over 30 Days for 4 kg
8 and 16
Maximum Allowable Volume
(mL) in One Blood Draw (2.5% of
Total Blood Volume)
Total Maximum Allowable Volume
(mL) Drawn Over 30 Days for 5 kg
10 and 20
Maximum Allowable Volume
(mL) in One Blood Draw (2.5% of
Total Blood Volume)
Total Maximum Allowable Volume
(mL) Drawn Over 30 Days for 6 kg
12 and 24