DLA 16

Question 1

How to study DNA in the lab?

Answer 1

1. enzymes
2. cloning
3. PCR
4. hybridization

Question 2

What do restriction endonucleases do?

Answer 2

They cut DNA within a segment of DNA

Question 3

What class of restriction endonuclease is most helpful in molecular biology

Answer 3

Class II

Question 4

What does class II restriction endonucleases do?

Answer 4

Cut DNA in a specific and reproducible fashion

helpful in DNA manipulation

Question 5

The four types of DNA poly used in the lab?

Answer 5

1. DNA polymerase I (exonuclease)
2. Klenow fragment DNA poly
3. reverse transcriptase (RNA as template)
4. Taq poly (need primers)

Question 6

What are three important features of cloning vectors

Answer 6

1. Can self-replicate
2. multiple cloning site (exonuclease region)
3. A selectable marker (antibiotic resistance)

Question 7

cloning vectors have what functions?

Answer 7

1. replication
2. sequencing
3. protein expression

Question 8

What is needed for PCR?

Answer 8

1. DNA poly
2. primers
3. dNTPs
4. magnesium chloride
5. Buffer
6. DNA