DNA

Question 1

1. Helicase

Answer 1

-main unzip
-2 single strand
-breaks the hydrogen bonds between the nitrogen bases

Question 2

2. single-stranded binding protein

Answer 2

-doorstop (cant zip up)
-bind to the single strands of DNA to the single strands of DNA to prevent rezipping

Question 3

3. Primase

Answer 3

binds to the single-strand DNA and lies down RNA primers to build new complementary strands

Question 4

4. DNA polymerase III

Answer 4

-hydrogen bond
-attaches nucleotides to one end of the growing complemtey strand and builds DNA in the 5’-3’
-reads from 3’-5’
-nucleotides lose two phosphates which generate the energy for this process

PRIMASE AND POLYMERASE III
-attach to neculotides on the lagging strands in short fragments (OKASAKI)

-replication continues in one direction - (Leading strand)
- DIscontinues in the other direction with (Lagging strand)

Question 5

5. DNA Polymerase I

Answer 5

-Replaces RNA primer with DNA neculotides

Question 6

6. Ligase

Answer 6

-joins the gaps in the lagging strand by building the sugar-phosphate backbone (phosphodiester linkage)