What is the characteristic DNA sequence (2 important ones) of the promotor region?
2 sequences:
- 25 TATA box (Hogness box)
- 75 CAAT box
What does the mutation in the DNA sequence cause???
Mutations in a promotor region cause less promotion… thus less transcription of these genes, thus decreases the amount of gene that is transcribed.
What structural motifs allow for proteins to bind to DNA?
- Helix-loop-helix
- Helix-turn-helix
- Zinc finger motif
- Leucine zipper protein
What are the different RNA polymerases in Eukaryotes? What about prokaryotes?
RNA Polymerase I - rRNA
RNA polymerase II- mRNA
RNA polymerase III- tRNA
Prokaryotes: 1 type of RNA polymerase, makes 3 types of RNA
What enzyme is responsible for tRNA charging?
Aminoacyl tRNA synthetase
What enzyme catalyzes peptide bond formation?
Peptidyl transferase
What enzyme is deficient in Lech-Nyhan syndrome? What is the TX?
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT)
Tx- Allopurinol
