DNA libraries

Question 1

DNA libraries

Answer 1

Set of cloned DNA fragments from a single source or organism

Question 2

Types of DNA libraries

Answer 2

• Whole genome (genomic library)
• Single chromosome (chromosome-specific library)
• Gene/set of genes that are expressed in a single cell type or at a certain time (cDNA library – transcriptome

Question 3

To make a genomic DNA library

Answer 3

1. chromosomal DNA is extracted.
2. It is digested with restriction enzymes, and the resulting fragments are inserted into vectors
3. Vector selection: the library ideally contains the whole genome in the smallest number of clones.
4. BACs and YACs used to accommodate the large sizes of DNA necessary to span the approximately 3 billion bp of human DNA (as in the Human Genome Project).

Question 4

Slide 15-19 part 1 and slide 4-9 of RecDNA- Es2