DNA Replication

Question 1

Where does DNA replication take place?

Answer 1

In the nucleus as DNA is too big to leave the nuclear pores

Question 2

Name the 4 steps of DNA replication

Answer 2

Replication fork formation
Initiation
Polymerase elongation
Termination

Question 3

Describe the 3 steps of RFF

Answer 3

1. DNA replication starts at the origin
2. Initiator proteins unzip double helix
3. DNA helicase breaks hydrogen bonds and unzips birdirectionally

Question 4

Describe the 4 stages of initiation.

Answer 4

1. 1 strand acts as a template
2. Short RNA primer attaches so that DNA polymerase can bind at 3’ end
3. DNA polymerase attaches at 3’ end and adds nucleotides
4. Forms phosphodiester bonds