DNA Replication

Question 1

describe the importance of DNA replication

Answer 1

• life begins as one cell and moves onward
• errors causes problems in cell division, which can lead to disease and possible death

Question 2

what is semiconservative replication

Answer 2

each daughter strand has half parent and half new DNA

Question 3

in what direction is new DNA made

Answer 3

5’ to 3’ direction

Question 4

how are both new DNA strands made in the 5’ to 3’ directions

Answer 4

through leading and lagging mechanisms

Question 5

what is the significance of DNA polymerase

Answer 5

• synthesizes new DNA
• monitors for correct base-pairing between incoming nucleotides
• proofreading/self-correcting

Question 6

describe the general process of DNA synthesis

Answer 6

• DNA polymerase needs base-paired nucleotides
• DNA primase makes small region of RNA for DNA polymerase to begin
• nuclease breaks RNA primer
• Repair polymerase replaces RNA with DNA
• DNA ligase joins in small grooves in DNA backbone

Question 7

what are SSBPs

Answer 7

• single-stranded binding proteins
• binds unwound single stranded DNA
• prevents base-pair formation

Question 8

what is the purpose of the sliding clamp

Answer 8

to keep DNA polymerase attached to template